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 Nucleic
acids, proteins and glycans are biopolymers widely distributed in living
organisms. All of these polymers are composed of covalently linked units but
glycans have characteristic features not found in nucleic acids and proteins.
Notably the nature of the linkage between the monomer units in glycans is more
variable than that found in the other biopolymers and this leads to a much
greater variety in the sequence of the biopolymer.
The
structural diversity of oligosaccharides found on glycoproteins and glycolipids
is enormous. This is due to the number of parameters that are varied in a
controlled way during assembly of an oligosaccharide.
Gene
technology and protein engineering, which have developed from knowledge obtained
by studies of nucleic acids and proteins, enable us to utilize living organisms
more effectively for the fermentation industry by directing them to produce a
particular protein. Thanks to these biotechnologies, we can now obtain
substantial amounts of bioactive proteins, which are useful but occur in very
minute amounts in animals. However, many proteins produced by animal cells occur
as glycoproteins. Because bacteria such as Escherichia coli lack
glycosylation machinery, recombinant proteins produced by them lack glycans.
Since many of these aglycosylated proteins do not express the expected
biological activities, functional roles of the glycans have recently been
attracting the interest of molecular biologists. On the basis of the idea of
elucidating the biological information contained in the glycans of
glycoconjugates and using them for the understanding of biology, the scientific
field called “Glycobiology” was established.
KRISHGEN
provides tools and solutions to the research, diagnostic
and biotech community for the analysis of these glycans and oligosaccharides...
please contact us ...
know
more by viewing our Resources Page
Analysis Tools
PNGase
F of glycosylated proteins
MALDI-TOF
of N-glycans and
purified proteins
ØHPLC
analysis of N-glycan
ØLarge
scale separation of N-glycans (P4
column)
Monosaccharides
compositional analysis
of N-glycans (HPAEC-PAD)
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