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Promotion & Discounts

 

* * * * * * *

Please note these promotion offers are valid only for India specific region only *

>> * Terms and Conditions Apply. <<

Promokine Cloning / Transfection Accessories Promotion Offer 2011

 

Order any Promokine Cloning / Transfection products & get a discount of 20% on our

Price List.

 

Order any products over Rs200,000 – & receive an original Olympus FE46 Digital
Camera FREE.

 

Plasmid Vectors

K-MB-P003100

pPK-CMV-E1 Expression Vector Kit

For high-level constitutive transgene expression

PK-MB-P003200

pPK-CMV-E2 Expression Vector Kit

For high-level constitutive transgene expression

PK-MB-P003300

pPK-CMV-E3 Expression Vector Kit

For high-level constitutive transgene expression

PK-MB-P003400

pPK-CMV-F1 Fusion Vector (C-GFP)

C-GFP-Fusion-Stable Reporter Vector

PK-MB-P013400

pPK-CMV-F2 Fusion Vector (N-GFP)

N-GFP-Fusion-Stable Reporter Vector

PK-MB-P003500

pPK-CMV-F3 Fusion Vector (C-Luc)

C-Luciferase-Fusion-Stable Reporter Vector

PK-MB-P013500

pPK-CMV-F4 Fusion Vector (N-Luc)

N-Luciferase-Fusion-Stable Reporter Vector

PK-MB-P000200

pPK-CMV-R0 Reporter Vector (Blank)

High-Level Transient Expression Vector Vector (blank)

PK-MB-P010200

pPK-CMV-R1 Reporter Vector (b-Gal)

High-Level Transient Reporter Vector (b-Gal)

PK-MB-P020200

pPK-CMV-R2 Reporter Vector (CAT)

High-Level Transient Reporter Vector (CAT)

PK-MB-P030200

pPK-CMV-R3 Reporter Vector (Luc)

High-Level Transient Reporter Vector (Luciferase)

PK-MB-P040200

pPK-CMV-R4 Reporter Vector (GFP)

High-Level Transient Reporter Vector (GFP)

PK-MB-P050200

pPK-CMV-R5 Reporter Vector (SEAP)

High-Level Transient Reporter Vector (SEAP)

 

PCR Cloning & Gene Expression Kits

PK-MB-FC003120

FastClone PCR Cloning Kit 1

Rapid PCR Cloning for Mammalian Gene Ex-pression

PK-MB-FC003220

FastClone PCR Cloning Kit 2

Rapid PCR Cloning for Mammalian Gene Ex-pression

PK-MB-FC003320

FastClone PCR Cloning Kit 3

Rapid PCR Cloning for Mammalian Gene Ex-pression

PK-MB-FE10110

FastExpress Gene Expression Kit

Rapid Mammalian Gene Expression

PK-MB-FE10210

FastExpress Gene Expression Kit

Rapid Mammalian Gene Expression

PK-MB-FE10120

FastExpress Gene Expression Kit (HA)

Rapid Mammalian Gene Expression

PK-MB-FE10220

FastExpress Gene Expression Kit (HA)

Rapid Mammalian Gene Expression

PK-MB-C101020

OptiCells Competent E. coli Cells

For optimal transformation results

 

Gene Silencing - siRNA Expression / Vector / Generation Kits

PK-MB-P100100

GeneOFF pPK-H1 shRNA Vector Kit

Highest-level expression of shRNA for gene

PK-MB-P100300

GeneOFF pPK-H1-GFP shRNA Vector Kit

Highest-level expression of shRNA for gene silencing

PK-MB-P600100

GeneOFF pPK-U6 shRNA Vector Kit

Highest-level expression of shRNA for gene

PK-MB-P600300

GeneOFF pPK-U6-GFP shRNA Vector Kit

Highest-level expression of shRNA for gene silencing

PK-MB-P140100

GeneOFF siRNA Expression Kit (H1)

Fast and effective way to produce siRNAs in

PK-MB-P140300

GeneOFF siRNA Expression Kit (H1-GFP)

Fast and effective way to produce siRNAs in vitro

PK-MB-P640100

GeneOFF siRNA Expression Kit (U6)

Fast and effective way to produce siRNAs in

PK-MB-P640300

GeneOFF siRNA Expression Kit (U6-GFP)

Fast and effective way to produce siRNAs in vitro

PK-MB-T510001

GeneOFF siRNA Generation Kit

Easy and economic generation of functional siRNAs

PK-MB-T520100

siProtect RNase Inhibitor

 

 

Order any products over Rs100,000 – and receive a MP4 player FREE.

 

Gene Booster

Enhancement of Gene Expression in Transfected Cells

The GeneBooster Reagent Kit is a set of chemical cocktails designed to significantly enhance gene expression

levels in transfected cells. Simply add GeneBooster reagent to the culture medium four hours post transfection

and up to 12 fold improvement in expression levels can be achieved. This kit can be used in combination with

all commercially available DNA transfection reagents (including our PromoFectin Reagents).
• Up to 12 fold increased gene expression levels in diverse cell lines post transfection
• Compatible with all commonly used cell transfection reagents
• Fast and easy protocol
• Optimized conditions for popular cell lines
• Efficient for both transient and stable transfections

 

CellClean

Highly Efficient CleanUp of Transfected Cells

CellClean Buffer is a unique washing buffer that has been designed to remove all complexes of DNA and

cationic lipids which associate with cell surfaces during transfection. It shows no cytotoxicity and can

distinguish between extracellular and intracellular plasmid DNA. When Cell-Clean Buffer is used as part

of the cell washing procedure, more precise quantitation of the amount of DNA delivered into cells can be

achieved. Moreover, it improves downstream results such as DNA stability, cytometry, and gene delivery

studies.
• Efficient removal of extracellular lipid/DNA complexes after cell transfection
• Non-toxic to cells
• Fast and easy procedure
• Better downstream results post transfection

PK-CT-2020-0

GeneBooster Reagent Kit (contains all 3 booster reagents)

Enhanced gene expression levels after cell transfection

PK-CT-2020-1

GeneBooster Reagent I

Enhanced gene expression levels after cell transfection

PK-CT-2020-2

GeneBooster Reagent II

Enhanced gene expression levels after cell transfection

PK-CT-2020-3

GeneBooster Reagent III

Enhanced gene expression levels after cell transfection

PK-CT-1000-01

CellClean Washing Buffer

Efficient cleanup of transfected cells

Dead or Alive?
You should know how your cells are doing!

 

 

How do your cells react when they are exposed to certain compounds or when essential factors are added or withdrawn? What happens when specific genes are knocked out and how cell metabolism is affected when foreign genes are expressed?

Check your cells’ condition and determine:

- Cell Viability & Proliferation
- Cell Senescence
- Cytotoxicity
- Cell Death & Apoptosis
- Cell Stress & Metabolism


PromoKine’s innovative cell-based assays provide sensitive, reliable and reproducible results with fast and convenient protocols.
                                                                    

Improve and simplify your cell testing: Order our products for Cell Analysis and Apoptosis Research and we will send you a free T-shirt for the onset of summer.*

*This offer is valid until June 30, 2011 and cannot be combined with other discounts.

Mesenchymal Stem Cells Tips and Tricks
 

Mesenchymal Stem Cells (MSCs) are fibroblastoid multipotent adult stem cells with a high capacity for self-renewal. Adult MSCs can be isolated from different tissues, e.g. bone marrow, adipose tissue, and umbilical cord matrix:
 

Human MSC from Bone Marrow
Human MSC from Umbilical Cord Matrix
Human MSC from Adipose Tissue


All of them can be expanded in culture while maintaining their multipotency (e.g. by using the PromoCell MSC Growth Medium) allowing production of large numbers of cells which then can be differentiated in vitro into a variety of tissue specific cells e.g. adipocytes, osteoblasts, myocytes, and chondrocytes.

MSC Differentiation
The directed differentiation of MSC allows the in vitro generation of functional biomaterials and is carried out by adding appropriate cytokines, such as those contained in PromoCell's ready-to-use MSC Differentiation Media. In some cases three-dimensional culture techniques and special ECM-mixtures are used for optimal support of this process. The terminally differentiated cells that form a tissue-like structure are identified by specific (immuno-)histochemical staining procedures.

PromoCell provides detailed application notes which describe the differentiation with PromoCell's MSC Differentiation Media and the subsequent staining process for Human MSCs:

Adipogenic Differentiation and Analysis of MSC
Chondrogenic Differentiation and Analysis of MSC
Neurogenic Differentiation and Analysis of MSC
Osteogenic Differentiation and Analysis of MSC


MSC Phenotype
Recently it was shown that MSC recruit from the perivascular niche which represents a tight network throughout the vasculature of the whole body [2]. These perivascular cells lack endothelial/hematopoietic markers (CD31, CD34) but express CD146, PDGF-R beta, and alkaline Phosphatase [2].
MSC show a typical CD10+, CD13+, CD44+, CD73+, CD105+ phenotype, but lack expression of CD31 or CD45 [1]. Antibodies and ELISA Kits for the identification of CD Antigen are available at PromoKine.

_________________________________________________________________
 

Get a free PromoCell T-Shirt with every order of PromoCell MSCs or the respective growth / differentiation media in the current Stem and Blood Cell Promotion !

 


___________________________________________________________________

*This offer is valid until June 30, 2011 and cannot be combined with other discounts.

 

Now with every bottle of   

Fetal Bovine Serum (500ml) ordered, get a free McDonalds voucher for Rs100-.

 

 

 

Offer Valid till December 31, 2011

 


 

 
   
     
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