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  SOLVO Biotechnology (Hungary) and KRISHGEN BioSystems (India) announce Distribution and Service Representation Agreement for Solvo’s Membrane Transporter Assay Products and Services in India, Singapore, Malaysia, Hong Kong, Thailand, Srilanka, Bangladesh, Pakistan and Nepal. Budapest, Hungary - Mumbai, India; 30th October, 2007

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  IDS (UK) announces exclusive distribution for India. The world's leading company in bone metabolism assays has recently taken over Nordic Biosciences, Denmark. These products are now available in India through KRISHGEN; 12th September, 2007. 

 

 

XENOBIOTICS

      

Subcellular Fractions

Xenotech standard products feature subcellular fractions from several different tissues (lung, kidney, intestine, and liver) and a variety of toxicologically-relevant species including: human, monkey, dog, rabbit, guinea pig, rat, hamster, and mouse. 

  • Microsomes   

  • S9  

  • Cytosol 

  • Mitochondria 

  • Reaction Phenotyping Kit  

Microsomes

XenoTech carries a large variety of microsomes from toxicologically relevant species: Human, Monkey, Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.

Liver microsomes (endoplasmic reticulum) are supplied at a concentration of 20 mg/mL, suspended in 250 mM sucrose.  This useful in vitro test system contains cytochromes P450, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases and other drug-metabolizing enzymes.

 

S9

The S9 fraction (post-mitochondrial supernatant fraction) is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of phase I and phase II enzymes including P450 enzymes, flavin-monooxygenases, carboxylesterases, epoxide hydrolase, UDP-glucuronosyltransferases, sulfotransferases, methyltransferases, acetyltransferases, glutathione S-transferases and other drug-metabolizing enzymes.

XenoTech carries a large variety of S9 from toxicologically relevant species: Human, Primate, Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.

 

Cytosol

Human liver cytosol (the soluble fraction) contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.

XenoTech prepare cytosol from toxicologically relevant species: Human, Primate, Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.  

 

Mitochondria

Human liver mitochondria contain monoamine oxidases A and B (MAO A and B), aldehyde dehydrogenases and other xenobiotic-metabolizing enzymes. Human liver mitochondria are supplied at a standard concentration of 20 mg/mL in 50 mM TRIS+HCl (pH 7.4 at 4?C) containing 150 mM KCl and 2 mM EDTA. Donor information is included with each order. This product is intended for in vitro studies of xenobiotic metabolism.

 

Reaction Phenotyping Kit

The Reaction Phenotyping Kit (RPK), Catalog number H0500, is designed to identify the human liver CYP enzyme(s) responsible for metabolizing a drug (or other xenobiotic), in order to predict pharmacokinetic variability, which can occur when a drug is metabolized by a polymorphically-expressed CYP enzyme. Samples in the kit are carefully selected to minimize correlations and avoid outliers that can interfere with reliable results.

 

Hepatocytes/Media

 Fresh Human Hepatocytes                                                   Cryopreserved Human Hepatocytes
 
Inducing Cryopreserved Human Hepatocytes                      Cryopreserved Primate Hepatocytes
 
Cryopreserved Dog Hepatocytes                                         Cryopreserved Rabbit Hepatocytes
 
Cryopreserved Rat Hepatocytes                                          Cryopreserved Mouse Hepatocytes
 
Hepatocyte Media/Kits

 

Cryopreserved Human Hepatocytes

XenoTech's cryopreserved human hepatocytes come in Assured Minimum Yield (AMY) Categories, so you can choose the number of viable cells that you need for your experiment.

 

Cryopreserved Primate Hepatocytes

Upon thawing, cryopreserved monkey hepatocytes remain viable and may be used in suspension for short-term drug metabolism experiments (we recommend incubations no longer than 6 hours). When cryopreserved monkey hepatocytes are thawed, isolated, and suspended under appropriate conditions, they can be used to evaluate the metabolism of drugs and NMEs.

 

Cryopreserved Dog Hepatocytes

Upon thawing, cryopreserved Beagle Dog hepatocytes remain viable and may be used in suspension for short-term drug metabolism experiments (we recommend incubations no longer than 6 hours). When cryopreserved Beagle Dog hepatocytes are thawed, isolated, and suspended under appropriate conditions, they can be used to evaluate the metabolism of drugs and NMEs.

 

Cryopreserved Rabbit Hepatocytes

Upon thawing, cryopreserved rabbit hepatocytes remain viable and may be used in suspension for short-term drug metabolism experiments (we recommend incubations no longer than 6 hours). When cryopreserved rabbit hepatocytes are thawed, isolated, and suspended under appropriate conditions, they can be used to evaluate the metabolism of drugs and NMEs.

 

Cryopreserved Mouse Hepatocytes

Upon thawing, cryopreserved mouse hepatocytes remain viable and may be used in suspension for short-term drug metabolism experiments (we recommend incubations no longer than 6 hours). When cryopreserved mouse hepatocytes are thawed, isolated, and suspended under appropriate conditions, they can be used to evaluate the metabolism of drugs and NMEs.

 

Cryopreserved Rat Hepatocytes

Upon thawing, cryopreserved rat hepatocytes remain viable and attach reliably to cell culture dishes in a manner similar to that observed with freshly isolated rat hepatocytes. When cultured under appropriate conditions, these cryopreserved hepatocytes can be used to evaluate drugs and NMEs as inducers of CYP enzymes. In addition, in vitro results on cryopreserved Sprague Dawley rat hepatocytes yield excellent correlation with in vivo results.

 

Hepatocyte Media/Kits

HEPATOCYTE INCUBATION KIT - K2500

50 mL of phenol red free Waymouths media Supplemented with the following:

i) Dexamethasone   ii) Fetal Bovine Serum   iii) Insulin   iv) Na+ Pyruvate    v) L-Glutamine

Hepatocyte isolation kits

These kits make thawing and isolation a snap.  Everything you need to prepare your cryopreserved hepatocytes for use. 

 

HEPATOCYTE RE-SUSPENSION MEDIA - K2200

When plating XenoTech's Inducible Cryopreserved Human Hepatocytes, it is necessary to resuspend the cell pellet in supplemented DMEM before putting cells in culture media. 40 mL per tube.

HEPATOCYTE CULTURE MEDIA - K2300

Supplemented Modified Chee's Medium, for plating/feeding Inducible Cryopreserved Human Hepatocytes, and recommended for use with fresh human hepatocytes. 100 mL per bottle.

 

   

Bactosomes
Bacterial membranes containing the following human cytochrome P450s coexpressed with human NADPH-cytochrome P450 reductase:

∙ CYP1A1   ∙ CYP1A2      ∙ CYP1B1      ∙ CYP2A6    ∙ CYP2A13   ∙ CYP2B6    ∙ CYP2C8    ∙ CYP4A11  ∙ CYP2C9   ∙ CYP2C18     ∙ CYP2C19    ∙ CYP2D6    ∙ CYP2E1     ∙ CYP2J2     ∙ CYP3A4    ∙ CYP3A5

 

EasyCYP

For the ultimate in convenience, selected CYPs are also available in our new EasyCYP format, offering standard CYP concentration, standard protein concentration, and a standard vial size throughout the range.

 

EasyCYP Bactosomes  

setting new standards for ease-of-use

ü          Standard CYP concentration    

        Standard protein concentration     

ü          Standard vial size

 

Bactosome Kits

Bactosome Kits contain one vial of each of the five most popular CYPs, together with a free vial of Control Bactosomes.

 

High Reductase Bactosome Kit                                      

∙ CYP1A2R Bactosomes       ∙ CYP2C9HR Bactosomes       ∙ CYP2C19R Bactosomes

∙ CYP2D6R Bactosomes       ∙ CYP3A4R Bactosomes          ∙ Control Bactosomes

 

Low Reductase Bactosome Kit                                                                                         

∙ CYP1A2LR Bactosomes       ∙ CYP2C9R Bactosomes       ∙ CYP2C19LR Bactosomes

∙ CYP2D6LR Bactosomes       ∙ CYP3A4LR Bactosomes      ∙ Control Bactosomes

 

 

SOLVO BIOTECHNOLOGY

 

PREDEASY ATPase Assay Kits:

It is a unique product for measuring the ATPase dependent Drug-ABC membrane transporter interaction in a “Ready to Assay” All-in-One Package !

Benefits of PREDEASY ATPase Assay Kit over conventional method:

> Higher Sensitivity                                     > More Robust                    > Easy to use

> Easier automation, Higher throughput         > More reliable                   > ALL-in-One

> Lower cost for each Data Point generated 

 

Membrane Products for ATPase and Vesicular Transporter Assays

1.        Membrane preparation for ATPase Assays

2.        Membrane preparation for vesicular transport Assays

 

ATPase Assays for ABC Transporters:

Membrane prepared from recombinant baculovirus infected Sf9 cells as well as from mammalian cell or tissue are widely used to detect the interaction of compounds with different ABC (ATP-binding cassette) transporters.

The ATPase Assays are used in two different modes:

       1.   ATPase activation and

       2.   ATPase inhibition

 

Vesicular Transport Assays for ABC Transporters:

Membranes prepared from ABC transporter over-expressing cells can be used to assay the interaction of test drugs with ABC transporters.

 

The Calcein Assay:

SOLVO’s patented calcein assay is an indirect inhibitory type whole cell assay, to provide information on any interaction between the ABC transporter and the test drug that would affect the transport of the reporter compound (fluorescence dye precursor).

“The Calcein Assay is a robust cell based screening assay that allows the identification and characterization of ABCB1 inhibitors at a medium to high throughput”

 

SOLVO products can be used to study the transport properties of these pharmacological barriers:

1. In vitro transporter assays for hepato-biliary DMPK      2. Transporter assays for enteral absorption PK

3. Transporter assay for blood-brain barrier                     4. In vitro transporter assay for renal excretion

 

The screening of the interaction of test drugs, nutrients and other molecules with ABC transporters is an excellent way to study their Hepato-Biliary DMPK and with uptake transporters is an excellent way to study their absorption and renal elimination.