|
Subcellular
Fractions
Xenotech
standard products feature subcellular fractions from several different tissues
(lung, kidney, intestine, and liver) and a variety of toxicologically-relevant
species including: human, monkey, dog, rabbit, guinea pig, rat, hamster, and
mouse.
-
Microsomes
-
S9
-
Cytosol
-
Mitochondria
-
Reaction
Phenotyping Kit
Microsomes
XenoTech
carries a large variety of microsomes from toxicologically relevant species: Human,
Monkey, Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.
Liver
microsomes (endoplasmic reticulum) are supplied at a concentration of 20 mg/mL,
suspended in 250 mM sucrose. This useful in vitro test system
contains cytochromes P450, flavin-monooxygenases, carboxylesterases, epoxide
hydrolase, UDP-glucuronosyltransferases and other drug-metabolizing enzymes.
S9
The
S9 fraction (post-mitochondrial supernatant fraction) is a mixture of microsomes
and cytosol. Accordingly, it contains a wide variety of phase I and phase II
enzymes including P450 enzymes, flavin-monooxygenases, carboxylesterases,
epoxide hydrolase, UDP-glucuronosyltransferases, sulfotransferases,
methyltransferases, acetyltransferases, glutathione S-transferases and other
drug-metabolizing enzymes.
XenoTech
carries a large variety of S9 from toxicologically relevant species: Human,
Primate,
Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.
Cytosol
Human
liver cytosol (the soluble fraction) contains many xenobiotic metabolizing
enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol
dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases,
sulfotransferases, glutathione S-transferases, etc.
XenoTech
prepare cytosol from toxicologically relevant species: Human,
Primate, Dog, Rat, Mouse, Rabbit, Guinea Pig, Hamster and Mini-pig.
Mitochondria
Human
liver mitochondria contain monoamine oxidases A and B (MAO A and B), aldehyde
dehydrogenases and other xenobiotic-metabolizing enzymes. Human liver
mitochondria are supplied at a standard concentration of 20 mg/mL in 50 mM
TRIS+HCl (pH 7.4 at 4?C) containing 150 mM KCl and 2 mM EDTA. Donor information
is included with each order. This product is intended for in vitro studies of
xenobiotic metabolism.
Reaction
Phenotyping Kit
The
Reaction Phenotyping Kit (RPK), Catalog number H0500, is designed to identify
the human liver CYP enzyme(s) responsible for metabolizing a drug (or other
xenobiotic), in order to predict pharmacokinetic variability, which can occur
when a drug is metabolized by a polymorphically-expressed CYP enzyme. Samples in
the kit are carefully selected to minimize correlations and avoid outliers that
can interfere with reliable results.
Hepatocytes/Media
 Fresh
Human Hepatocytes
Cryopreserved
Human Hepatocytes
Inducing
Cryopreserved Human Hepatocytes
Cryopreserved
Primate Hepatocytes
Cryopreserved
Dog Hepatocytes
Cryopreserved
Rabbit Hepatocytes
Cryopreserved
Rat Hepatocytes
Cryopreserved
Mouse Hepatocytes
Hepatocyte
Media/Kits
Cryopreserved
Human Hepatocytes
XenoTech's
cryopreserved human hepatocytes come in Assured Minimum Yield (AMY) Categories,
so you can choose the number of viable cells that you need for your experiment.
Cryopreserved
Primate Hepatocytes
Upon
thawing, cryopreserved monkey hepatocytes remain viable and may be used in
suspension for short-term drug metabolism experiments (we recommend incubations
no longer than 6 hours). When cryopreserved monkey hepatocytes are thawed,
isolated, and suspended under appropriate conditions, they can be used to
evaluate the metabolism of drugs and NMEs.
Cryopreserved
Dog Hepatocytes
Upon
thawing, cryopreserved Beagle Dog hepatocytes remain viable and may be used in
suspension for short-term drug metabolism experiments (we recommend incubations
no longer than 6 hours). When cryopreserved Beagle Dog hepatocytes are thawed,
isolated, and suspended under appropriate conditions, they can be used to
evaluate the metabolism of drugs and NMEs.
Cryopreserved
Rabbit Hepatocytes
Upon
thawing, cryopreserved rabbit hepatocytes remain viable and may be used in
suspension for short-term drug metabolism experiments (we recommend incubations
no longer than 6 hours). When cryopreserved rabbit hepatocytes are thawed,
isolated, and suspended under appropriate conditions, they can be used to
evaluate the metabolism of drugs and NMEs.
Cryopreserved
Mouse Hepatocytes
Upon
thawing, cryopreserved mouse hepatocytes remain viable and may be used in
suspension for short-term drug metabolism experiments (we recommend incubations
no longer than 6 hours). When cryopreserved mouse hepatocytes are thawed,
isolated, and suspended under appropriate conditions, they can be used to
evaluate the metabolism of drugs and NMEs.
Cryopreserved
Rat Hepatocytes
Upon
thawing, cryopreserved rat hepatocytes remain viable and attach reliably to cell
culture dishes in a manner similar to that observed with freshly isolated rat
hepatocytes. When cultured under appropriate conditions, these cryopreserved
hepatocytes can be used to evaluate drugs and NMEs as inducers of CYP enzymes.
In addition, in vitro results on cryopreserved Sprague Dawley rat hepatocytes
yield excellent correlation with in vivo results.
Hepatocyte
Media/Kits
HEPATOCYTE
INCUBATION KIT - K2500
50
mL of phenol red free Waymouths media Supplemented with the following:
i)
Dexamethasone ii) Fetal Bovine
Serum iii) Insulin
iv) Na+ Pyruvate v) L-Glutamine
Hepatocyte
isolation kits
These
kits make thawing and isolation a snap. Everything you need to prepare
your cryopreserved hepatocytes for use.
HEPATOCYTE
RE-SUSPENSION MEDIA - K2200
When
plating XenoTech's Inducible
Cryopreserved Human Hepatocytes,
it is necessary to resuspend the cell pellet in supplemented DMEM before putting
cells in culture media. 40 mL per tube.
HEPATOCYTE
CULTURE MEDIA - K2300
Supplemented
Modified Chee's Medium, for plating/feeding Inducible Cryopreserved Human
Hepatocytes, and recommended for use with fresh
human hepatocytes.
100 mL per bottle.
Bactosomes
Bacterial membranes containing the following human cytochrome P450s coexpressed
with human NADPH-cytochrome P450 reductase:
∙
CYP1A1 ∙ CYP1A2
∙ CYP1B1 ∙ CYP2A6
∙ CYP2A13 ∙
CYP2B6 ∙ CYP2C8 ∙ CYP4A11
∙ CYP2C9 ∙
CYP2C18 ∙
CYP2C19 ∙ CYP2D6 ∙ CYP2E1
∙ CYP2J2 ∙
CYP3A4 ∙ CYP3A5
EasyCYP
For
the ultimate in convenience, selected CYPs are also available in our new EasyCYP
format, offering standard CYP concentration, standard protein concentration, and
a standard vial size throughout the range.
EasyCYP
Bactosomes
setting
new standards for ease-of-use
ü
Standard CYP concentration
Standard protein concentration
ü
Standard vial size
Bactosome
Kits
Bactosome
Kits contain one vial of each of the five most popular CYPs, together with a
free vial of Control Bactosomes.
High
Reductase Bactosome Kit
∙
CYP1A2R Bactosomes
∙ CYP2C9HR Bactosomes
∙ CYP2C19R Bactosomes
∙
CYP2D6R Bactosomes
∙ CYP3A4R Bactosomes
∙ Control Bactosomes
Low
Reductase Bactosome Kit
∙
CYP1A2LR Bactosomes
∙ CYP2C9R Bactosomes
∙ CYP2C19LR Bactosomes
∙
CYP2D6LR Bactosomes
∙ CYP3A4LR Bactosomes
∙ Control Bactosomes
SOLVO
BIOTECHNOLOGY
PREDEASY
ATPase Assay Kits:
It
is a unique product for measuring the ATPase dependent Drug-ABC membrane
transporter interaction in a “Ready to Assay” All-in-One Package !
Benefits
of PREDEASY ATPase Assay Kit over conventional method:
>
Higher Sensitivity
> More Robust
> Easy to use
>
Easier automation, Higher throughput
> More reliable
> ALL-in-One
>
Lower cost for each Data Point generated
Membrane
Products for ATPase and Vesicular Transporter Assays
1.
Membrane preparation for ATPase Assays
2.
Membrane
preparation for vesicular transport Assays
ATPase
Assays for ABC Transporters:
Membrane
prepared from recombinant baculovirus infected Sf9 cells as well as from
mammalian cell or tissue are widely used to detect the interaction of compounds
with different ABC (ATP-binding cassette) transporters.
The
ATPase Assays are used in two different modes:
1. ATPase activation and
2. ATPase inhibition
Vesicular
Transport Assays for ABC Transporters:
Membranes
prepared from ABC transporter over-expressing cells can be used to assay the
interaction of test drugs with ABC transporters.
The
Calcein Assay:
SOLVO’s
patented calcein assay is an indirect inhibitory type whole cell assay, to
provide information on any interaction between the ABC transporter and the test
drug that would affect the transport of the reporter compound (fluorescence dye
precursor).
“The
Calcein Assay is a robust cell based screening assay that allows the
identification and characterization of ABCB1 inhibitors at a medium to high
throughput”
SOLVO
products can be used to study the transport properties of these pharmacological
barriers:
1.
In vitro transporter assays for hepato-biliary DMPK
2. Transporter assays for enteral absorption PK
3.
Transporter assay for blood-brain barrier
4. In vitro transporter assay for renal excretion
The
screening of the interaction of test drugs, nutrients and other molecules with
ABC transporters is an excellent way to study their Hepato-Biliary DMPK and with
uptake transporters is an excellent way to study their absorption and renal
elimination.
|
