ATPase Assay Kits

Benefits of PREDEASY ATPase Assay Kit over conventional method:
> Higher Sensitivity                                    

> More Robust                   

> Easy to use
> Easier automation, Higher throughput        

> More reliable                  

> ALL-in-One
> Lower cost for each Data Point generated 

Membrane Products for ATPase & Vesicular Transporter Assays

• Membrane preparation for ATPase Assays
• Membrane preparation for vesicular transport Assays

ATPase Assays for ABC Transporters:
Membrane prepared from recombinant baculovirus infected Sf9 cells as well as from mammalian cell or tissue are widely used to detect the interaction of compounds with different ABC (ATP-binding cassette) transporters.

The ATPase Assays are used in two different modes:

1. ATPase activation and
2. ATPase inhibition

Vesicular Transport Assays for ABC Transporters :
Membranes prepared from ABC transporter over-expressing cells can be used to assay the interaction of test drugs with ABC transporters.

The Calcein Assay :
SOLVO’s patented calcein assay is an indirect inhibitory type whole cell assay, to provide information on any interaction between the ABC transporter and the test drug that would affect the transport of the reporter compound (fluorescence dye precursor).

“The Calcein Assay is a robust cell based screening assay that allows the identification and characterization of ABCB1 inhibitors at a medium to high throughput”

SOLVO products can be used to study the transport properties of these pharmacological barriers:
1. In vitro transporter assays for hepato-biliary DMPK     

2. Transporter assays for enteral absorption PK
3. Transporter assay for blood-brain barrier                    

4. In vitro transporter assay for renal excretion

The screening of the interaction of test drugs, nutrients and other molecules with ABC transporters is an excellent way to study their Hepato-Biliary DMPK and with uptake transporters is an excellent way to study their absorption and renal elimination.