The classic assay format is based on PBMC-derived macrophages as effector cells. After extraction of fresh human PBMCs, monocytes will be isolated and differentiated in culture to macrophages. The workflow is much more sophisticated than ADCC and usually takes more than one-week period. Phagocytosis events will be analyzed using FACS screening, and a dose-dependent curve will be generated to assess the ADCP potency in detail. Of note, effector cells from distinct donors cannot be pooled due to MHC restrictions. Hence, samples from a battery of donors must be applied to reduce donor-specific variability.
However, this is resolved today with off-the-shelf macrophages now available with Certificate of Analysis standardizing their isolation in cryopreserved formats.
No tedious steps
Easy-to-use Macrophages now available !!!
Primary ADCP assay can be a powerful tool for early phage confirmation of in-vivo ADCP liability, as well as for biocompatibility assessment of biosimilars and biobetters.
The challenge to offer macrophages or do the isolation in-house led to alternate methods including reporter-gene based assays which are expensive to perform and still do not reflect the true MOA (mechanism of action) as they use engineered cell lines.
Macrophage-based ADCP assay can reflect the ADCP MOA with improved specificity, sensitivity and accuracy. Our fast, accurate, reproducible and cost-effective platform using off-the-shelf macrophages can help deliver in vivo ADCP bioactivities as well as lot release and stability evaluation.
Human M1 Macrophages (GM-CSF), monocyte-derived
Human M2 Macrophages (M-CSF), monocyte-derived
Primary human macrophages are hard to isolate from tissue in sufficient amounts with a homogeneous phenotype, do not proliferate in culture, and are difficult to handle in vitro.
Krishgen’s PromoCell cryopreserved human monocyte-derived macrophages (hMDM) provide a convenient and easy-to-handle solution. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks.
Cryopreserved human macrophages are produced from human monocytes in well-proven M1/M2- Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) or M2- (M-CSF) polarized cells. Each cryo-vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.
Assay-ready: Fully functional, adherent M1/M2 macrophages
Save time and start your experiments immediately with a defined cell number
Wide range of donors available
Each lot is tested for: CD68+, CD80+ (M1); CD68+, CD163+ (M2)
View Flyer : ADCP Assays – Cryo Hu Macrophages
Call us to learn more or email your queries at: firstname.lastname@example.org
|Catalog No||Product Name||Pack Size|
|C-12914||Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor||1,5 Mio cryopreserved cells|
|C-12916||Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor||5 Mio cryopreserved cells|
|C-12915||Human M2 Macrophages (M-CSF), Monocyte-derived, single donor||1,5 Mio cryopreserved cells|
|C-12917||Human M2 Macrophages (M-CSF), Monocyte-derived, single donor||5 Mio cryopreserved cells|