Antibody Purification & Modification Systems
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect™ Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to 500µg of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link™ conjugation system (available separately), which allows the purified antibody to be rapidly labeled with HRP, AP or RPE.
Maleimide Activated Labels
Maleimide activated proteins react readily with molecules that contain sulfhydryl (thiol) groups to create a stable thioether linkage. Innova's activated proteins are supplied as a stable freeze dried formulation; all materials are rigorously QC tested using thiolated biomolecules to ensure high efficiency conjugation.
Chemiluminescent & ELISA Components
Chemiluminescent substrates are characterized by varying lengths of light emission and intensity, allowing you to choose desired sensitivity levels for your assay.
TMB substrates, BSA and Fish Gelatin Blocks, DAB, HRP-Conjugate stabilizers, Secondary Antibodies etc. are available from Medicago, Sweden's leading company and other companies.
Innova Biosciences, UK
Bioconjugation Reagents & Kits
|Easy Conjugation Kits (Lighting-Link Kits)|
|Lightning-Link Cy3 / Cy3.5 / Cy5 / Cy5.5 / Cy7|
|Lightning-Link Tandem PE/Cy5|
|Lightning-Link Tandem PE/Cy5.5|
|Lightning-Link Tandem PerCP/Cy5.5|
|Lightning-Link Atto390 / 425 / 465 / 488 / 532 / 565 / 594 / 611 / 620 / 633 / 655 / 680 / 700|
|Antibody Purification and Modification Systems|
|AbSelect Antibody Purification Kit|
|Innovative Products for Drug Discovery and Proteomics|
|PiColorlock™ Assay Reagent|
|Phosphate Detection Reagents for Measuring Phosphatases, ATPases and Nucleotidases|
|ATPase / GTPase Assay Kits|
|Nucleotide Affinity Resins|
|γ-Phosphate-Linked ATP-Agarose (KinaseBind™)|