Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed.Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2, G1 and G2 and it is the analyte with the highest toxic significance. Thin-layer chromatography has been a common method of aflatoxin detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of aflatoxin B1 ELISA kit to analyze aflatoxin B1 residue of samples is fast and accurate. Refer to the National Standard GB / T 5009.22-2003.
Principle: The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with aflatoxin antigen. Free aflatoxin in sample solutions and the immobilized antigen compete for aflatoxin enzyme conjugate. Any unbound enzyme conjugate is then removed in a washing step. Substrate/chromogen is added to the wells, bound enzyme conjugate converts the chromogen into a blue product. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorbance is inversely proportional to the aflatoxin concentration in the sample.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
|Pack Size -||
1 x 96 wells
Rapid Multiple Immunoenzyme Assay of Mycotoxins
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Rapid Immunoenzyme Assay of Aflatoxin B1 Using Magnetic Nanoparticles
Alexandr E. Urusov, Alina V. Petrakova, Maxim V. Vozniak, Anatoly V. Zherdev and Boris B. Dzantiev
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