Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed. AF M1 is the hydroxylated metabolite of aflatoxin B1, also a strong carcinogen. Milk and its products are one of the food susceptible to aflatoxin M1 contamination.The methods to test Aflatoxin M1 residue is high-performance liquid chromatography (HPLC),thin layer chromatography (TLC) etc. The use of this AF M1 ELISA kit to analyze Aflatoxin M1 residue of samples is fast and accurate.
Principle: The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with aflatoxin M1 antigen. Free aflatoxin M1 in sample solutions and the immobilized antigen compete for aflatoxin enzyme conjugate. While AF M1 antibody and HRP secondary antibody (HRP material) combined, via the TMB substrate show color, sample absorbance values of aflatoxin M1 has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw aflatoxin M1 content in samples.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
1 x 96 wells
The product has not yet been referenced specifically in any publications.
Publishing research using this product? Please let us know so that we can cite the reference for this product.
Our Inside Sales Team is available to assist, should a problem arise with your order. Please inspect your packages/goods immediately upon receipt and notify us promptly of any damage or discrepancies. Should an item be shipped to you incorrectly, as the result of an error on our part, we will take quick and appropriate action to correct the problem.
Before returning any reagents or goods, please contact us to obtain an authorization and shipping instructions. A return authorization will ensure the safe and proper handling of material and enable us to expedite a resolution. Items returned without prior authorization may not be accepted. Kindly note for certain specific products like primary cell lines, hepatocytes, microsomes, tissues where the storage is below -20°C, we reserve the right not to accept any returns. Shipment of authorized returns should be made within 3 days of confirmation received from us.
We will do our best to fulfil requests to return material. However, in order to maintain the quality of our products and continue to provide competitive prices, certain items may not be returned. These items include: diagnostic reagents; refrigerated or frozen products; reagents and standards which have passed their expiration dates; custom products or special offers; products missing labels, parts, or instruction manuals; and books, computer software and equipment removed from their original packaging. Returns accepted for items ordered in error may be subject to a 30% processing fee and all returns must be returned within 10 days of purchase.