Anti Cetuximab (ERBITUX) ELISA
Cetuximab (Erbitux) is an epidermal growth factor receptor (EGFR) inhibitor used for the treatment of metastatic colorectal cancer and head and neck cancer. Cetuximab is a chimeric (mouse/human) monoclonal antibody given by intravenous infusion. In July 2009, the FDA approved Erbitux for treatment of KRAS wild type colon cancer, since Cetuximab had little or no effect in colorectal tumors harboring a KRAS mutation.
Intended Use: This Anti Cetuximab ELISA is a rapid and easy method for the qualitative screening of antibodies to Cetuximab in human serum and plasma. The Anti Cetuximab is for research use only. Not for diagnostic or therapeutic procedures.
Principle: The method employs the qualitative sandwich enzyme immunoassay technique. A commercially available Erbitux is pre-coated onto microwells. Samples and Controls are pipetted into microwells and antibodies to human Cetuximab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated Erbitux is pipette and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of antibodies to Cetuximab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
ELISA 96 wells
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