Description
Background: Colistin is a polypeptide antibiotic, which is commonly applied in curing sensitive organism infection and promoting growth of pup and poultry. It is employed as medicine feed additives and inhibitor of Gram-negative bacteria such as bacillus pyocyaneus and Escherichia coli. Colistin is excreted by kidney, and it can cause kidney epithclium denaturalization. The common approach to detect colistin is microbiology inhibition assay. Due tothe big molar weight, strong polarity and thermal instability, it is inappropriate to detect colistin with GC, thus the sensitivity and detection limit is restricted. Enzyme immunoassay is more precise and sensitive, and simpler operating, and can considerably minimized work intensity.
Principle: KRIBIOLISA Colimycin ELISA kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Colistin residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme conjugate, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the colistin reside in it, after comparing with the Standard Curve, multiplied by the Dilution factor, colistin residue quantity in the sample can be calculated.
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