Anti – Bird Flu/Avian Influenza Virus(H5N1) ELISA
Bird flu (Avian influenza, AI) is an acute contact venereal toxicity infectious disease against the domestic poultry industry at present. Vaccination is the most effective way to prevent and control it. The antibody level after immune reflects the vaccine effect, which directly related to immune resistance to avian influenza virus in chicken flocks. This product is applicable to the different species, different age in chicken serum specific antibody detection of avian influenza virus. It can be used for avian influenza (virus) vaccine immune time of analysis, evaluation of immune effect, chickens with avian influenza in immune status.
Intended Use: This kit is used to detect Bird Flu (H5N1) virus IgG antibodies in chicken serum or plasma.
Principle: The Bird Flu (H5N1) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
1 x 96 wells
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