Description
Introduction:
Glatiramer Acetate (Copaxone) is a mixture of random-sized peptides that are composed of the four amino acids found in myelin basic protein, namely glutamic acid, lysine, alanine, and tyrosine. Myelin basic protein is the antigen in the myelin sheaths of the neurons that stimulates an autoimmune reaction in people with multiple sclerosis (MS), so the peptide may work as a decoy for the attacking immune cells.
Intended Use:
The KRIBIOLISA Glatiramer Acetate ELISA kit is used for the quantitative estimation of Glatiramer Acetate in cell culture supernatant and pharmaceutical preparations.
Principle:
The Glatiramer Acetate ELISA is a competitive immunoassay for the determination of Glatiramer Acetate. A varying concentration of unlabeled standard or sample and constant concentration of GA:HRP conjugate will bind in sequence to the antibodies coated on the microplate. Upon washing, unbound GA:HRP Conjugate will be removed. Bound GA:HRP complex will produce a soluble blue colored product after the addition of TMB Substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Glatiramer Acetate present in the standards or samples.
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