Vasculogenesis in the embryo is the process by which new blood vessels are generated de novo from primitive precursor cells. Angiogenesis is the process of new blood vessel formation from pre-existing vasculatures. It plays an essential role in development, normal tissue growth, wound healing, the female reproductive cycle (placental development, ovulation, corpus luteum) and also plays a major role in various diseases. One of the key molecules for angiogenesis and for the survival of the endothelium is vascular endothelial growth factor (VEGF).
Intended Use: Mouse VEGF ELISA is specifically designed for the accurate quantitation of Mouse VEGF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive. This Mouse VEGF is for research use only. Not for diagnostic or therapeutic procedures.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
ELISA 96 wells
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