Description
Background: Neomycin is an aminoglycoside antibiotic that fights bacteria in the body. Neomycin is used to reduce the risk of infection during surgery of your intestines. Due to the causes of anaphylactic reaction and resistance, in EU, US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one detection, so it can considerably minimize operation error and work intensity.
Principle: KRIBIOLISA Neomycin ELISA kit is based on indirect-competitive ELISA. The microtiter wells are coated with antigen. Neomycin residue in the sample competes with antigen coated on the microtitre plate for the antibody. After the addition of enzyme conjugate immune complex will form. After washing, addition of TMB Substrate is used to show the color. Absorbance of the sample is negatively related to the Neomycin residue in it, after comparing with the Standard Curve, multiplied by the dilution factor, Neomycin residue in the sample can be calculated.
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