Description
Introduction:
Oxyntomodulin (Glucagon37) is a 37-amino acid polypeptide, consisting of the glucagon molecule plus a Cterminal extension. It is produced by ileal L cells after cleavage of proglucagon by prohormone convertase 1; mice lacking prohormone convertase 1 have a reduction in oxyntomodulin, GLP-1, GLP-2, and glicentin levels. As with GLP-1, oxyntomodulin is inactivated by DPP-4. Endogenous oxyntomodulin appears to work as a satiety signal, rising after a meal
Intended Use:
The Oxyntomodulin ELISA is used for estimation of Oxyntomodulin in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes.
Principle:
The Oxyntomodulin ELISA is a competitive immunoassay for the determination of Oxyntomodulin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Oxyntomodulin bound.
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