Description
Background: Benzyl penicillin (BP) or Penicillin G is a broadly applied antibiotic, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU,US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one detection, so it can considerably minimize operation error and work intensity. This ELISA can be used in the production of therapeutic drugs and vaccines, to accurately quantify drug residues and ensure quality and purity of the final product. Current method of drug residue testing such as HPLC or GC-MS are with sample prep not only complex and expensive, but also difficult and time consuming. ELISA speed up and simplify the analysis without losing accuracy.
Principle: KRIBIOLISA Penicillin ELISA kit is based on indirect-competitive ELISA. The microtiter wells are coated with antigen. Penicillin residue in the sample competes with antigen coated on the microtitre plate for the antibody. After the addition of enzyme conjugate immune complex will form. After washing, addition of TMB substrate is used to show the color. Absorbance of the sample is negatively related to the Penicillin residue in it,
after comparing with the Standard Curve, multiplied by the dilution factor, Penicillin residue in the sample can be
calculated.
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