Protein A ELISA
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use.
Intended Use: This Protein A kit is used for determination of natural and recombinant constructs of Protein A ligand contamination, in various antibody products that are purified through Protein A chromatographic techniques. This protein A is for research use only. Not for diagnostic or therapeutic procedures.
Principle: This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with antiProtein A:HRP antibody simultaneously in the microtitre wells already coated with affinity purified capture antiProtein A antibody. This immunological reaction results in formation of a sandwich complex of solid phase antibody-Protein-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure (see Assay procedure section mentioned below). The substrate 3,3’,5,5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtitre plate reader and it is directly proportional to the concentration of Protein A present.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
ELISA 96 wells
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