Tumor necrosis factor-alpha (TNF-α), a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further, TNF-α has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF functions as a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines.
Intended Use: Rat TNF-alpha ELISA is specifically designed for the accurate quantitation of Rat TNF-alpha from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive. This Rat TNF-alpha is for research use only. Not for diagnostic or therapeutic procedures.
The kit uses a sandwich ELISA format to quantify the target cytokine between two layers of antibodies (i.e. capture and detection antibody). The cytokine to be measured contains at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive upto pg/ml levels (more sensitive than direct or indirect ELISA). A highly efficient blocking and stabilizing proprietary solution is used in the kit. All KRISHGEN cytokine ELISA kits are optimized to give the best possible noise:signal ratios.
KRISHGEN BIOSYSTEMS is a leading manufacturer of immunoassay kits including ELISA. KRISHGEN has over 200 publications and citations to its credit which speaks of its quality and accuracy. Leaders in many areas of research, ELISAs manufactured by KRISHGEN are used for quality research and reproducibility at competitive prices. Diverse product range includes ELISA for diagnostics, cytokines, biopharmaceuticals, HCP, downstream contamination, PK studies for Mabs, immunogenicity, vaccine, animal health testing, plant pathology, GMO and food safety.
ELISA 96 wells
Poly-ingredient Formulation Bresol® Ameliorates Experimental Chronic Obstructive Pulmonary Disease (COPD) in Rats
Sci Pharm. Volume 81, ISSN 3, 2013, pp 833-42.
Mohamed Rafiq*, Viswanatha GOLLAPALLE LAKSHMINARAYANASHASTRY, Suryakanth DATTATRAY ANTURLIKAR, Jagadeesh MAHALINGAIAH, Dhanush KRISHNA, Pralhad SADASHIV PATKI
Department of Pharmacology, R&D Center, The Himalaya Drug Company, Makali, Bangalore, Karnataka.
*Corresponding author - firstname.lastname@example.org (Mohamed Rafiq)
Activity profile of methanolic extract of Ficus bengalensis: Comparison with some clinically effective drugs
Biomedicine & Aging Pathology 2014
Atul T. Thite, Rajesh R. Patil, Suresh Ramnath Naik Corresponding author contact information, E-mail the corresponding author
Investigations on leucas cephalotes (roth.) Spreng. For inhibition of Lps-induced pro-inflammatory mediators in murine macrophages and in Rat model
EXCLI Journal 2015
Spermidine ameliorates 3-nitropropionic acid (3-NP)-induced striatal toxicity: Possible role of oxidative stress, neuroinflammation, and neurotransmitters
Physiology and Behavior 2015
Sumit Jamwala, Puneet Kumara
Oleic Acid Induced Acute Lung Injury/Acute Respiratory Distress Syndrome Animal Model
Research Journal of Pharmacy and Technology 2014
Hagawane Tejashree N.1, Mahuvakar Aashish M., Gaikwad Rajiv V., Kshirsagar Nilima A.
Beneficial effects of lycopene against haloperidol induced orofacial dyskinesia in rats: Possible neurotransmitters and neuroinflammation modulation
European Journal of Pharmacology 2016
Swati Datta, Sumit Jamwal, Rahul Deshmukh, Puneet Kumar
Development of subcutaneous sustained release nanoparticles encapsulating low molecular weight heparin
Journal Of Advanced Pharmaceutical Tech. and Research 2015
Satheesh Jogala, Shyam Sunder Rachamalla, and Jithan Aukunuru
Ameliorative effects of Tricholepis glaberrima in experimentally induced hepatic damage in rats: Modulation of cytokines functions
Journal of Ethnopharmacology 2015
Shilpa S Gound, Vishnu N. Thakare, Sharjil Khan, Raju R. Wadekar, Suresh R. Naik
Neuroprotective Activity of Curcumin in Combination with Piperine against Quinolinic Acid Induced Neurodegeneration in Rats
Singh S., Kumar P.
Beneficial effect of rice bran extract against 3-nitropropionic acid induced experimental Huntington's disease in rats
Navneet Kaur, Sumit Jamwal, Rahul Deshmukh, Vinod Gauttam, Puneet Kumar
Protective Effect of Spermidine Against Excitotoxic Neuronal Death Induced by Quinolinic Acid in Rats: Possible Neurotransmitters and Neuroinflammatory Mechanism
Neurotoxicity Research 2015
Sumit Jamwal, Shamsher Singh, Navneet Kaur, Puneet Kumar
Piperine Enhances the Protective Effect of Curcumin Against 3-NP Induced Neurotoxicity: Possible Neurotransmitters Modulation Mechanism
Neurochemical Research 2015
Shamsher Singh, Sumit Jamwal, Puneet Kumar
Alleviation of the hepatotoxic effect of bromobenzene by the Indian traditional herbal formulation Triphala in experimental rats
Oriental Pharmacy and Experimental Medicine 2014
Udhaya Lavinya Baskaran, Mahaboobkhan Rasool, Evan Prince Sabina
Amelioration of bromobenzene hepatotoxicity by Withania somnifera pretreatment: Role of mitochondrial oxidative stress
Toxicology Reports 2014
Mahima Vedi, Mahaboobkhan Rasool, Evan Prince Sabina
An experimental study to investigate the impact of p-coumaric acid, a common dietary polyphenol, on cadmium chloride-induced renal toxicity
Food and Function 2014
Dhanalakshmi Navaneethan and Mahaboob Khan Rasool
Intratracheal (IT) Lipopolysaccharide (LPS) Induced Acute Lung Injury (ALI)/Acute Respiratory Distress Syndrome (ARDS) in Wistar Rats
Research Journal of Pharmacy and Technology 2014
Hagawane Tejashree N., Mahuvakar Aashish M., Gaikwad Rajiv V., Kshirsagar Nilima A
L-theanine, a Component of Green Tea Prevents 3-Nitropropionic Acid (3-NP)-Induced Striatal Toxicity by Modulating Nitric Oxide Pathway
Molecular Neurobiology 2016
Sumit Jamwal, Puneet Kumar
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