Description
Background:
Axatilimab (SNDX-6352) is a humanized IgG4 antibody with high affinity to CSF-1R. Axatilimab can be used for the research of chronic graft versus host disease (cGVHD) and neoplastic diseases.
Intended Use:
For Estimation of Axatilimab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Axatilimab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Axatilimab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Axatilimab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Axatilimab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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