GENLISA™ Human SARS-CoV-2 Spike Protein Antigen ELISA (Qualitative)

SKU: KBVH015-4 Category:

42,000.00

ELISA for the qualitative determiantion of SARS-CoV-2 (Covid-19) Spike protein antigen in respiratory specimens and serum. CE marked for diagnostic use.


Availability: 2 – 3 Days | Pack Size: 1 x 96 wells



Description

Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Intended Use:
The GENLISA Human SARS-CoV-2 (Covid-19) Spike Protein Antigen ELISA kit is used as an analytical tool for qualitative antigen determination of Human SARS-CoV-2 (Covid-19) spike proteins in human serum and plasma and cell culture supernatant.

Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and Controls are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.










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Additional information

Species

Human

Sample Type

Serum and Plasma

Calibration Range

Qualitative

Detection Method

Colorimetric, 450nm

Regulatory Status

For Research Use Only

Research Area

COVID-19

Citations

1. Tinko Eftimov , Petia Genova-Kalou , Georgi Dyankov , Wojtek J Bock , Vihar Mankov , Sanaz Shoar Ghaffari , Petar Veselinov , Alla Arapova , Somayeh Makouei Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study Biosensors (Basel).