Description
Background:
Panitumumab, is a IgG2 human monoclonal antibody specific to the epidermal growth factor receptor. EGFR is a transmembrane protein. Panitumumab works by binding to the extracellular domain of the EGFR preventing its activation. This results in halting of the cascade of intracellular signals dependent on this receptor.
Intended Use:
For Estimation of Panitumumab (VECTIBIX) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Panitumumab antibody and Panitumumab present in the sample and standards are bound by Anti- Panitumumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Panitumumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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