KRIBIOLISA Trastuzumab (HERCEPTIN) ELISA
The Trastuzumab (Herceptin) ELISA kit is intended for the quantitative determination of Trastuzumab (Herceptin) in serum, plasma and cell culture samples. CE IVD marked.
- Product Specifications
This Trastuzumab ELISA kit is for quantitative testing of Trastuzumab in serum, plasma and cell culture samples. Trastuzumab is a monoclonal antibody used primarily to treat breast cancer that is Her2 receptive. It is also sold under its brand name Herceptin. Trastuzumab is primarily administered when there is amplification of the HER2 oncogene or overexpression of the protein in the tumour. Increased activation of the HER2 triggers multiple downstream pathways that lead to abnormal cancer cell proliferation. Trastuzumab binds to the HER2, suppressing cell growth and proliferation.
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Trastuzumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Trastuzumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Trastuzumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Trastuzumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
|Storage Condition||2-8 Deg C|
|Usage||For In-Vitro Diagnostic Use Only|