- Product Specifications
This Cetuximab ELISA is for determination of Cetuximab in human serum, plasma and cell culture supernatant. Cetuximab is a recombinant chimeric monoclonal antibody, used for the treatments of cancer of the colorectal region, non-small cell lung cancer and head and neck cancer. Cetuximab, an EGFR inhibitor, is sold under the trade name Erbitux.
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Cetuximab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Cetuximab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Cetuximab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Cetuximab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
|Other Specifications||Sample Type: Serum, cell cuture supernatant and plasma|
|Calibrator Range||10 - 400ng/ml|
|Storage Condition||2-8 Deg C|
|Usage||For In-Vitro Diagnostic Use Only|