Gene Therapy has emerged as a promising future to disease treatment and personalised therapies. Gene Therapy is the introduction or modification of genes that modifies gene function to prevent or cure diseases.
AAV based Gene Therapy
In vivo gene therapy involves direct delivery of a therapeutic gene into the target cell in a patient's body. This can be carried out by both viral and non viral vectors, though the most popular emergence in vectors has been the Adeno-Associated Virus vector (AAV). AAV binds to a cellular receptor, enters the cell, migrates to the nucleus, and delivers a single-stranded DNA genome to establish a latent state for long-term gene expression in the absence of coinfection with a helper virus.
AAV vectors are commonly used in optogenetics experiments. These viruses are preferred over lentiviruses because they remain primarily episomal, while lentiviruses integrate into the genome. This is important because local chromatin structure at the site of genome integration can affect the expression of transgenes, as well as the expression of neighboring genes. Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV5. These serotypes differ in their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing specific cell types.
Krishgen has a range of titration AAV ELISA kits for a rapid and reliable way of quantifying AAV vectors based on a monoclonal antibody sandwich technique. And a range of Anti-AAV ELISA kits for qualitative determination of antibodies to AAV5 in serum and plasma.
DCs represent unique antigen-producing cells capable of sensitizing T cells to antigens. They play a central role in the regulation of innate and adaptive immunity. DC therapy represents a new and promising immunotherapeutic approach for treatment of different types of cancer.
Dr Jayaraman D. Lead Scientist, leading CRO company
I have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply Chain
The expertise and command on the logistics chain including the regulatory
guidelines from Krishgen is something we admire. As they often indicate, let us
do the supplies, and let your team focus on science … is really something the
company works on. Delivering consistently, with less than 1% error or delay
consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UK
To have a company understand science and work with them, is a pleasure. Krishgen
not only sells but understands what it sells in terms of the complex cutting
Mellisa Karlowski, Lead Researcher, east coast US CRO
The company is good on understanding its customers, is transparent and
speaks science. This makes it easier for us to work with them.