KRISHGEN is the leading provider of tools used by the biopharmaceutical industry. We provide assays and reagents used for drug development from upstream monitoring of glycosylation to downstream impurities and contamination assays to bioanalytical assays for protein characterization as per FDA/EMA. We also offer some of the world’s only kits for pharmacokinetic studies and for post marketing surveillance for immunogenicity testing.
Antibodies for your Bioassays - PK / Immunogenicity - Bridging | Ligand Binding | Neutralizing
KRISHGEN offers highly specific, high affinity anti-biotherapeutic antibodies to support preclinical research, clinical trials and patient monitoring for innovator and biosimilar products from leading companies Bio-Rad, Sino Biologicals and Krishgen. Biorad uses the HuCAL recombinant monoclonal antibody library and an improved method of antibody phage display to generate these fully human antibodies in Fab and full immunoglobulin formats
Sino offers quality antibodies for developing your assays maintaining quality and consistency. Leading biosimilar companies in India procure these antibodies from us for their biosimilar assays.
We are uniquely placed that when we sell these antibodies, we are also major users of these antibodies as we use them to develop our own assays which are commercially offered as ready-to-use ELISA kits.
Anti-idiotypic antibodies are able to recognize and bind to the idiotype of another antibody. KRISHGEN has various solutions to problems that you may encounter during the development of anti-idiotype antibodies and its assays for biocharacterization.
Anti-idiotype antibodies are widely used in therapeutic drugs studies:
Antibody drug immunogenicity studies
Anti-drug antibodies (ADA) for clinical development
Antibody drug pharmacokinetic/pharmacodynamics (PK/PD) studies: detecting free, total or bound drug in blood
Bio-Rad is one of the world’s largest antibody producer supplying thousands of catalog antibodies and proteins. They include isotype specific secondary antibodies, phosphor-specific Precision antibodies, fluorescent western blotting antibodies and others including idiotypic antibodies for biopharma assays.
The efficacy of a therapeutic antibody is dependent on the Fab fragment and its binding activity to the target antigen, as well as the Fc fragment and its interaction with key Fc receptors. The binding affinity of the Fc fragment towards FcRn (FCGRT
The classic assay format is based on PBMC-derived macrophages as effector cells. After extraction of fresh human PBMCs, monocytes will be isolated and differentiated in culture to macrophages. The workflow is much more sophisticated than ADCC and usually takes more than one-week period. Phagocytosis events will be analyzed using FACS screening, and a dose-dependent curve will be generated to assess the ADCP potency in detail. Of note, effector cells from distinct donors cannot be pooled due to MHC restrictions. Hence, samples from a battery of donors must be applied to reduce donor-specific variability.
However, this is resolved today with off-the-shelf macrophages now available with Certificate of Analysis standardizing their isolation in cryopreserved formats.
No tedious steps
Easy-to-use Macrophages now available !!!
Primary ADCP assay can be a powerful tool for early phage confirmation of in-vivo ADCP liability, as well as for biocompatibility assessment of biosimilars and biobetters.
The challenge to offer macrophages or do the isolation in-house led to alternate methods including reporter-gene based assays which are expensive to perform and still do not reflect the true MOA (mechanism of action) as they use engineered cell lines.
Macrophage-based ADCP assay can reflect the ADCP MOA with improved specificity, sensitivity, accuracy; it can also be established in quality control, drug development settings. Our fast, accurate, reproducible and cost-effective platform can deliver compelling data regarding in vivo ADCP bioactivities as well as lot release and stability evaluation.
Human M1 Macrophages (GM-CSF), monocyte-derived
Human M2 Macrophages (M-CSF), monocyte-derived
Primary human macrophages are hard to isolate from tissue in sufficient amounts with a homogeneous phenotype, do not proliferate in culture, and are difficult to handle in vitro.
Krishgen’s cryopreserved human monocyte-derived macrophages (hMDM) provide a convenient and easy-to-handle solution. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks.
Cryopreserved human macrophages are produced from human monocytes in well-proven M1/M2- Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) or M2- (M-CSF) polarized cells. Each cryo-vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.
Dr Jayaraman D. Lead Scientist, leading CRO company
I have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology as we were working on an insulin analog. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply Chain
The expertise and command on the logistics chain including the regulatory
guidelines from Krishgen is something we admire. As they often indicate, let us
do the supplies, and let your team focus on science … is really something the
company works on. Delivering consistently, with less than 1% error or delay
consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UK
To have a company understand science and work with them, is a pleasure. Krishgen
not only sells but understands what it sells in terms of the complex cutting
Mellisa Karlowski, Lead Researcher, east coast US CRO
The company is good on understanding its customers, is transparent and
speaks science. This makes it easier for us to work with them.