CRISPR/Cas9 technique has revolutionized the field of sequence-specific gene editing due to its high precision, simplicity and flexibility. The CRISPR/Cas9 system comprises of the:
sequence-specific guide RNA (gRNA).
They can form a very stable ribonucleoprotein (RNP) complex. A specific DNA region is matched with the gRNA carrying Cas9 resulting in the target DNA getting cleaved with a double stranded break. The Cas9 RNP complex can be introduced into cells directly. It acts immediately after it enters the cell without transcription and translation. By matching the gRNA sequence with different functional domains, the CRISPR/Cas9 system and its variants have been used for a broad range of gene editing applications (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).
KRISHGEN offers a range of tools to assemble your own Cas9 and gRNA constructs, as well as purified Cas9 enzymes, antibodies for in vitro and in vivo use.
Gene Editing Kits
gRNA activity screening
Mutation detection kit
SpCas9 (mAb Rb, mouse)
FnCpf1, AsCpf1, LbCpf1
All-in-one or dual SpCas9, Nickase & SaCas9
Constructs for gRNA Expression
Constructs for Transcription Activation
Synthetic Cas9 crRNAs
Synthetic Cas9 tracrRNAs
Libraries for genome-wide knock-out
Libraries for genome-scale transcription activation
Dr Jayaraman D. Lead Scientist, leading CRO company
I have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology as we were working on an insulin analog. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply Chain
The expertise and command on the logistics chain including the regulatory
guidelines from Krishgen is something we admire. As they often indicate, let us
do the supplies, and let your team focus on science … is really something the
company works on. Delivering consistently, with less than 1% error or delay
consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UK
To have a company understand science and work with them, is a pleasure. Krishgen
not only sells but understands what it sells in terms of the complex cutting
Mellisa Karlowski, Lead Researcher, east coast US CRO
The company is good on understanding its customers, is transparent and
speaks science. This makes it easier for us to work with them.