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CRISPR/Cas9 Products

CRISPR/Cas9 technique has revolutionized the field of sequence-specific gene editing due to its high precision, simplicity and flexibility. The CRISPR/Cas9 system comprises of : 

1) the Cas9 nuclease 

2) the sequence-specific guide RNA (gRNA). 

They can form a very stable ribonucleoprotein (RNP) complex. A specific DNA region is matched with the gRNA carrying Cas9 resulting in the target DNA getting cleaved with a double stranded break. The Cas9 RNP complex can be introduced into cells directly. It acts immediately after it enters the cell without transcription and translation. By matching the gRNA sequence with different functional domains, the CRISPR/Cas9 system and its variants have been used for a broad range of gene editing applications (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).

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