STRs are important in current, forensic DNA testing. The abbreviation, STR stands for Short Tandem Repeat. STRs are the type of DNA used in most of the currently popular forensic DNA tests. STR is a generic term that describes any short, repeating DNA sequence.
One of the more commonly encountered STR test designs in forensic testing is Multiplex STR. Such systems combine three or more different PCRs in one reaction that targets distinct STR loci at the same time. Three of the commonly used loci are called, CSF1PO, TPOX and THO1.
Multiplex STRs are often combined with PCR for another locus called, Amelogenin (adds little to the discriminating power of the test). Its purpose is to help distinguish male and female sources of DNA by detecting the X and Y chromosomes. The Amelogenin products have sizes that place them outside the size ranges of the other loci.
Multiplex STRs are technically more simple and direct at the allele detection stage. On the other hand, multiplex STR is slightly more vulnerable to missing alleles. There are two reasons for this.
Larger DNA fragments are degraded before smaller ones. This is simply due to the fact that larger DNA molecules are bigger targets for degradative enzymes than smaller DNA molecules.
PCR itself favors (will produce more of) smaller DNA targets compared to larger ones that take more time to copy. The copying is done by a protein called an enzyme. It can finish copying smaller DNA fragments more rapidly than larger ones.
We have focused on developing assays under the DHARMAPLEX brand. Lord Yudhistra, is the eldest brother of the five Pandavas in the epic Indian lore - the Mahabharata . He was known for his righteousness, respect of elders, and honesty. He was also known as Dharmaraja - the King of Justice. We strive to aim that our DharmaPlex Forensic kits will be aid towards “dharma” and “nyaya”, meaning justice.
Dr Jayaraman D. Lead Scientist, leading CRO company
I have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology as we were working on an insulin analog. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply Chain
The expertise and command on the logistics chain including the regulatory
guidelines from Krishgen is something we admire. As they often indicate, let us
do the supplies, and let your team focus on science … is really something the
company works on. Delivering consistently, with less than 1% error or delay
consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UK
To have a company understand science and work with them, is a pleasure. Krishgen
not only sells but understands what it sells in terms of the complex cutting
Mellisa Karlowski, Lead Researcher, east coast US CRO
The company is good on understanding its customers, is transparent and
speaks science. This makes it easier for us to work with them.