Primer/Probe Set aligned as per CDC, Atlanta, USA guidelines.
Uses Taqman probes. Taqman Probes are widely used RT-PCR assay because of its sensitivity and specificity with dual quenchers for higher accuracy.
With 2/3 Primer/Probe option
Compared to other real-time PCR methods, we use fewer probes, which are labeled with the same fluorophore, guaranteeing that this assay can be used for detection in a single fluorescent channel or can be run on single-channel instruments.
THE PRIMER/PROBE IN THE KITS ARE TAGGED WITH FAM REPORTER DYE AND USE DOUBLE QUENCHERS FOR HIGH ACCURACY. The FAM DYE has an advantage that it can be used in any channel in majority of the Real Time PCR Instruments commercially available in the market. Please see a list of compatible instruments with the KBRT010 SARS-CoV-2 Real Time PCR Kit below.