In vitro safety testing for chemicals, cosmetics, household products, pharmaceutical, medical devices and textiles:
Krishgen Biosystems offers skin irritation and Ocular irritation test that have been endorsed by the ECVAM Scientific Advisory Committee (ESAC) as validated in vitro replacement for the Draize Skin / Ocular Irritation Test – OECD Test guidelines 439. Skin irritation tests are performed using in vitro 3D human reconstructed skin tissue models developed by Episkin.
ADVANTAGES OF IN-VITRO DERMAL AND OCULAR IRRITATION TESTING
An ethical method
Carried out in vitro, the SkinEthic reconstructed Epidermis and In Vitro international Irritection method makes it possible to evaluate the irritant potential of chemical products, without using animal testing.
The use of a suitable standardized model
Compared to in vivo study, the SkinEthic reconstructed epidermis and Irritaction assay that allows use of measurement systems that produces quantitative, reproducible results.
A validated method
The ECVAM validation of the method has shown its reproducibility within and between laboratories, and demonstrated that it is rapid and economical.
Superior technical expertise.
Rapid, reliable and cost effective testing.
Tests carried out as per specific guidelines.
Advanced data analysis software, data storage and analysis.
Well-equipped Laboratory with fully automated sample testing systems.
Starting Price (per sample)
Sample quantity required
Nature of Sample
Dermal Irritation assay
4 – 5 Weeks
40 mg or 40 ul (for triplicate run)
Solid, liquid, cream, gel etc.
4 – 5 Weeks
50 mg or 50 ul (for triplicate run)
Solid : 2gm
Solid, Liq etc.
Liquid: 2 ml
Ocular Irritation Assay
Solid : 2gm
Liquid: 2 ml
Extra charges applicable if sample needs additional pretreatment.
Extra charges applicable for rapid turnaround time.
Please enquire us for more details.
The MTT cytotoxicity assay:
A simple robust test for evaluating cellular viability following application of an irritant/toxic agent. The validated method enables evaluation of the viability of epidermal samples after a set time in contact with the product.
IL – 1α release (optional with extra charges)
IL1 alpha measurement in culture media increases the sensibility of the test. Products can thus be classified according to EU and OECD norms.
Dr Jayaraman D. Lead Scientist, leading CRO company
I have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply Chain
The expertise and command on the logistics chain including the regulatory
guidelines from Krishgen is something we admire. As they often indicate, let us
do the supplies, and let your team focus on science … is really something the
company works on. Delivering consistently, with less than 1% error or delay
consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UK
To have a company understand science and work with them, is a pleasure. Krishgen
not only sells but understands what it sells in terms of the complex cutting
Mellisa Karlowski, Lead Researcher, east coast US CRO
The company is good on understanding its customers, is transparent and
speaks science. This makes it easier for us to work with them.