In vitro safety testing for chemicals, cosmetics, household products, pharmaceutical, medical devices and textiles:
Krishgen Biosystems offers skin irritation and Ocular irritation test that have been endorsed by the ECVAM Scientific Advisory Committee (ESAC) as validated in vitro replacement for the Draize Skin / Ocular Irritation Test – OECD Test guidelines 439. Skin irritation tests are performed using in vitro 3D human reconstructed skin tissue models developed by Episkin.
An ethical method
Carried out in vitro, the SkinEthic reconstructed Epidermis and In Vitro international Irritection method makes it possible to evaluate the irritant potential of chemical products, without using animal testing.
The use of a suitable standardized model
Compared to in vivo study, the SkinEthic reconstructed epidermis and Irritaction assay that allows use of measurement systems that produces quantitative, reproducible results.
A validated method
The ECVAM validation of the method has shown its reproducibility within and between laboratories, and demonstrated that it is rapid and economical.
|Test||Model||Starting Price (per sample)||Turnaround time||Sample quantity required||Nature of Sample|
|Dermal Irritation assay||RHE||Contact us||4 – 5 Weeks||40 mg or 40 ul (for triplicate run)||Solid, liquid, cream, gel etc.|
|Episkin||Contact us||4 – 5 Weeks||50 mg or 50 ul (for triplicate run)|
|Irritection Assay||Contact us||2 weeks||Solid : 2gm||Solid, Liq etc.|
|Liquid: 2 ml|
|Ocular Irritation Assay||Contact us||2 weeks||Solid : 2gm|
|Liquid: 2 ml|
The MTT cytotoxicity assay:
A simple robust test for evaluating cellular viability following application of an irritant/toxic agent. The validated method enables evaluation of the viability of epidermal samples after a set time in contact with the product.
IL – 1α release (optional with extra charges)
IL1 alpha measurement in culture media increases the sensibility of the test. Products can thus be classified according to EU and OECD norms.
Dr Jayaraman D. Lead Scientist, leading CRO companyI have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply ChainThe expertise and command on the logistics chain including the regulatory guidelines from Krishgen is something we admire. As they often indicate, let us do the supplies, and let your team focus on science … is really something the company works on. Delivering consistently, with less than 1% error or delay consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UKTo have a company understand science and work with them, is a pleasure. Krishgen not only sells but understands what it sells in terms of the complex cutting edge sceince.
Mellisa Karlowski, Lead Researcher, east coast US CROThe company is good on understanding its customers, is transparent and speaks science. This makes it easier for us to work with them.
ARE YOU A
Join KRISHGEN as a verified distributor to get access to over 1 million sku’s, including reagents, biochemicals and assays. Your one stop vendor for life sciences.