Mycoplasma contamination is a significant problem to the culture of cells / cell lines used for research. Mycoplasma can cause devastating effects on eukaryotic cells as they can modify the cellular parameter leading to variable experimental results and potentially unsafe biological products. Mycoplasmas cannot be detected by visual inspection using a normal light microscope and thus, can remain unnoticed in the cell cultures for long periods. This makes the testing for Mycoplasma essential to ensure reliable research and reproducibility of data.
|Test||Test type||Sample volume required||Nature of sample||Turnaround Time||Starting price per sample (in duplicates) (in INR)|
|Mycoplasma testing Service||Conventional PCR||Minimum 1 ml||Cell culture supernatant||3-5 days after receipt of the sample||Rs. 16,910|
Culture suspension or adherent cells, primary cells, virus stocks and other biological can be tested. Culture supernatants are the preferred sample material.
Polymerase Chain Reaction is the most commonly used technique used for detecting Mycoplasma. The Mycoplasma DNA is isolated and amplified in vitro by DNA polymerase using specific primers to generate adequate amount of target, which is visualized on the agarose gel for detection.
The primer used in this assay amplifies the highly conserved 16S rRNA region of the Mycoplasma genome. The detection spectrum includes Mycoplasma orale, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma argininii, Mycoplasma salivarium, Mycoplasma hominis, Acholeplasma laidlawii and many other species. However, this assay does not detect the clinically relevant species Mycoplasma pneumoniae and Ureaplasma urealyticum which are not known as cell culture contaminants. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct 479 bp band derived from the internal control DNA on the agarose gel.
The detection limit is approx. 10-15 fg of Mycoplasma DNA.
The result is reported as Detected or Not Detected.
The results are reported 48 to 72 hrs from receipt of the samples.
|TERMS AND CONDITIONS FOR SAMPLE ACCEPTANCE:|
|Samples are to be shipped frozen (dry ice).|
|Sample should be clear and non-hemolyzed.|
|Samples should be properly packed and parafilm sealed in falcon tubes or vials.|
|Any sample that is tampered or which does not adhere to the above terms on receipt will not be processed further.|
Dr Jayaraman D. Lead Scientist, leading CRO companyI have been working with a team from KRISHGEN on an assay development and customization before giving us. The project was complicated and needed technical expertise in immunology. I was very pleased with their technical competence and also mastering immunology and delivering a tough project to us on time. Their rigor, punctuality and expertise have been a recipe for the high standards, I would expect for such project from them. Kudos, Krishgen Team!!!
Mr Ramesh Sravanan, VP-Supply ChainThe expertise and command on the logistics chain including the regulatory guidelines from Krishgen is something we admire. As they often indicate, let us do the supplies, and let your team focus on science … is really something the company works on. Delivering consistently, with less than 1% error or delay consistently is the key to their success.
Dr Keith Stevens, visiting Scientist, Norfolk University, UKTo have a company understand science and work with them, is a pleasure. Krishgen not only sells but understands what it sells in terms of the complex cutting edge sceince.
Mellisa Karlowski, Lead Researcher, east coast US CROThe company is good on understanding its customers, is transparent and speaks science. This makes it easier for us to work with them.
ARE YOU A
Join KRISHGEN as a verified distributor to get access to over 1 million sku’s, including reagents, biochemicals and assays. Your one stop vendor for life sciences.