Description
Introduction:
Fertirelin is one of the most potent synthetic LH-RH analogs inducing the release of luteinizing hormone and follicle-stimulating hormone from the anterior pituitary gland. It is a therapeutic agent for the prophylaxis and/or treatment of cancer, an infectious disease, a fibrotic disease, an inflammatory disease, a neurodegenerative disease, an autoimmune disease, or a heart and vascular disease.
Intended Use:
The Calcitonin ELISA is used for estimation of Calcitonin in serum, plasma, in pharmacokinetics, peptide delivery study and other purposes.
Principle:
The Fertirelin ELISA is a competitive immunoassay for the determination of Fertirelin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Fertirelin bound.
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