KRIBIOLISA™ Liraglutide (Victoza) ELISA

SKU: KBI5020 Category:

47,000.00

Enzyme Immunoassay for the quantitative estimation of Liraglutide in human serum and plasma.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 4 – 5 days | Pack Size: 1 x 96 wells



Description

Introduction:
Liraglutide (NN2211) is a derivative of a human incretin (metabolic hormone), glucagon-like peptide-1 (GLP-1) that is used as a long-acting glucagon-like peptide-1 receptor agonist, binding to the same receptors as does the endogenous metabolic hormone GLP-1 that stimulates insulin secretion. Marketed under the brand name Victoza, it is an injectable drug developed by Novo Nordisk for the treatment of type 2 diabetes. In 2015, Novo Nordisk began marketing a separate strength in the U.S. and E.U. under the brand name Saxenda as a treatment for adults who are obese or overweight with at least one weight-related comorbid condition.

Intended Use:
The KRIBIOLISA Liraglutide (Victoza/Saxenda)ELISA is used as for the quantitative determination of Liraglutide in human serum and plasma.

Principle:
The method employs a sandwich immunoassay for the determination of Liraglutide. The anti-Liraglutide Antibodies are coated on microtiter plate. Liraglutide standard and Liraglutide present in the samples will bind to coating antibody. Anti-GLP-1 antibody conjugated to HRP is then added which produces a soluble colored product after addition of TMB substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of bound Liraglutide present in the standards or samples.

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Additional information

Species

Human

Sample Type

Serum, Plasma, or Tissue extracts

Calibration Range

40 ng/ml – 2560 ng/ml

Sensitivity

less than 40ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Diabetes / Metabolism

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.

Citations